김길우의 논문 이야기1(2010.08)

글쓴이: 중원대 국제생명공학연구소 김길우 (☎02, 3408-2203)

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Title: The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice

금번 논문은 <Experimental Parasitology>에 실린 실험논문으로, 캘리포니아 의대의 이형우 박사와 하버드 의대의 김형환 박사, 중원대학교의 강윤중, 김도경 박사, 상지대 손영주 박사와 함께 쓴 논문입니다. 임팩트팩터는 1.773이며, 전염병학에서는 폭넓은 인용과 활용도를 자랑하는 우수한 논문집입니다.

Abstract: To develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein Pvs28 was cloned from Korean malaria patients using polymerase chain reaction. The Pvs28 gene consists of 726 bp and encods 241 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, rPvs28, has a molecular

weight of about 28 kDa in SDS-PAGE analysis. A monoclonal antibody against rPvs28 was produced using BALB/c mice. It inhibited sporozoite development in Anopheles sinensis mosquitoes (n=81) which are known as the main malaria transmission vector in Korea. Its antibody titer persisted past six months at relatively high titers. These results indicate that rPvs28 can induce an immune response in mice that effectively blocks sporozoite development in mosquitoes. Additionally, rPvs28 induces Th1/Th2 immune responses. Therefore it might be a useful vaccine candidate for preventing vivax malaria in Korea.

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