글쓴이: 중원대 국제생명공학연구소 김길우 (☎02, 3408-2203)

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오늘은 작년 8월에 발표된 논문 소식 전해드립니다. 

Title: Blocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in  Anopheles sinensis

금번 논문은 <Clinical And Vaccine Immunology>에 실린 실험논문으로 플로리다 대학의 이형우 박사와 하버드 의대의 김형환 박사, 중원대 의료공학부의 김도경 박사와 함께 쓴 논문입니다. 본 저널의 임팩트팩터는 2.373입니다.

Abstract: To develope a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface 
protein Pvs25 was cloned from a Korean malaria patient. The Pvs25  gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquito Anopheles sinensis, which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.
by 유당선생 김길우(혁) 2011.01.17 14:35